High-performance liquid chromatography (2 units, one of which is equipped with a fraction collector)

High-performance liquid chromatography (2 units, one of which is equipped with a fraction collector)

Manufacturer: Agilent Technologies
Model: 1260 Infinity

Features

  • Organic biomolecule quantitative analyses (sugars, acids, polymers, etc.)
  • Simultaneous analysis (variable, depending on the type of analyte)
  • Capacity to analyze 1 to 10 samples per hour (99 sample carousel tray)
  • Detection limit ranges from 500 ppm to 20 000 ppm depending on the molecule)
  • Sensitivity of the device: 1 mAU (diode array detector) and 1 nRIU (refractive index detector)
  • Column C3, C8, C18, Amide C-16, NH2, exclusion gel
  • Simultaneous double detector: refractive index (sugars) and diode array (UV-visible)
  • Fraction collectors; a 15 four-millilitre bottle plate and a 40 two-millilitre bottle plate

Samples

  • Liquids
  • Sample size: 0.5 – 1.5 mL
  • Injection: 10-50 µL

Examples of applications

  • Fermentation bioprocess monitoring (biomolecule production, substrate consumption)
  • Monitoring and analysis of organic compounds in membrane filtration processes
  • Monitoring and analysis of harmful biomass recycling
  • Food component analyses
  • Analyses and quantification of the organic impurities of formulations
  • Separation, identification, and purification of organic molecules (fraction collector)
  • Analyses of the effectiveness of nanoparticle treatment on a variety of biomolecules
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