Gradient PCR and Q-PCR (molecular biology platform)
Manufacturer: Eppendorf
Model: Realplex4, Vapoprotect, and Mastercycler
Features
- Block and lids with a capacity for very rapid heating and cooling (PCR and qPCR)
- Independent gradient thermal function programming spread over 12 columns (PCR and qPCR)
- Gradient range of over 20 °C (PCR and qPCR), from 4 to 99 °C
- Vapo.protect technology reducing evaporation from the wells (qPCR)
- LED lights emit blue light at 470 nm and excite fluorophores
- (SYBR®Green, FAM, VIC, TET, HEX, ROX, JOE, TAMRA) (qPCR)
- Possibility to choose primers and probes such as Taqman, Beacon, and SYBR (qPCR)
- Continuous monitoring of DNA amplification and post-amplification analysis software
Examples of applications
- Amplification of target genes in various microorganisms (PCR)
- Detection and quantification of target genes (ex. proteins, enzyme) of a variety of microorganisms (qPCR)
- Monitoring bacterial population in bioprocesses (qPCR)
- Monitoring of gene expression using ARNm and genotype variation
- Optimization of process conditions through pathogen detection (presence/absence)