Gradient PCR and Q-PCR (molecular biology platform)

Gradient PCR and Q-PCR (molecular biology platform)

Manufacturer: Eppendorf
Model: Realplex4, Vapoprotect, and Mastercycler

Features

  • Block and lids with a capacity for very rapid heating and cooling (PCR and qPCR)
  • Independent gradient thermal function programming spread over 12 columns (PCR and qPCR)
  • Gradient range of over 20 °C (PCR and qPCR), from 4 to 99 °C
  • Vapo.protect technology reducing evaporation from the wells (qPCR)
  • LED lights emit blue light at 470 nm and excite fluorophores
  • (SYBR®Green, FAM, VIC, TET, HEX, ROX, JOE, TAMRA) (qPCR)
  • Possibility to choose primers and probes such as Taqman, Beacon, and SYBR (qPCR)
  • Continuous monitoring of DNA amplification and post-amplification analysis software

Examples of applications

  • Amplification of target genes in various microorganisms (PCR)
  • Detection and quantification of target genes (ex. proteins, enzyme) of a variety of microorganisms (qPCR)
  • Monitoring bacterial population in bioprocesses (qPCR)
  • Monitoring of gene expression using ARNm and genotype variation
  • Optimization of process conditions through pathogen detection (presence/absence)
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